讲座题目： Proteomics of neurodegenerative diseases
主讲嘉宾：Ka Wan Li, 荷兰阿姆斯特丹自由大学分子与细胞神经生物学系
Dementias pose major socio-medical burden, currently there is still no cure. Novel proteomics approaches hold promise to identify alterations of the brain proteome that could provide clues to the disease etiology. Here, we report our proteomics findings on human post-mortem brains affected with (1) Alzheimer’s Disease across Braak’s stages 0-6, and (2) their interaction with ageing; (3) the analysis of single cell types with granulovacuolar degeneration versus cells with neurofibrillary tangles; and (4) AD tissues with capillary cerebral amyloid angiopathy. We further studied (5) Frontotemporal Dementia with mutations of C9orf72, MAPT or PGRN. Brain slices and single cells identified by immunostaining were collected by laser-microdissection, and processed for quantitative proteomics analysis by data dependent and data independent LC-MS/MS analysis. These studies confirmed known cellular changes, and identified novel proteins that may underlie distinct aspects of the diseases, some of which hold promise to be developed as biomarker.
Dr. Ka Wan Li currently works at the Department of Neurosciences-CNCR, Vrije University Amsterdam. He is the group leader of Neuroproteomics at Vrije University Amsterdam. Ka Wan does research in Neuroscience. His current research focus on the characterization of protein nano-machineries that underlies synapse function and plasticity, and study synapse/tissue proteome changes in neurodegenerative and neuropsychiatric disorders. State-of-the-art quantitative proteomics techniques are employed, using both human post-mortem brain tissues from patients and healthy controls, and the diverse mouse models of brain disorders.